Products LumeGen & CardioGen: Pulsatile Pressure & Flow Bioreactor System

Watch a video of the LumeGen bioreactor

BISS's series of bioreactors provide mechanical stimulation to three dimensional tissue engineered constructs to create physiological conditions in-vitro. The patented LumeGen & CardioGen systems (US Patent # 7348175) impart pulsatile flow and pressure to a vascular conduit. Applications include the investigation of cell function and differentiation, pharmaceutical benchtop testing, and the seeding and growth of engineered tissues and medical devices.

LumeGen Chambers

The LumeGen bioreactor chamber creates pulsatile and shear stress stimulation for a single vascular construct. Standard construct sizes may range from 2 to 6mm inner diameter and from 10 to 60mm in length. The modular design facilitates scaffold installation and cell seeding separate from the stimulation system. Multiple ports are available for media access, catheter probe insertion, sample collection, or further instrumentation. The autoclavable chamber has a volume of 60mL. Additional chamber features include:

Design:
Polycarbonate removable windows for easy viewing and laser micrometer measurement
Size:
90mm(3.6”)L x 46mm(1.6”)W x 20mm(0.8”)D
Ports:
5

 

LumeGen Grip Options

The LumeGen chambers use barbed stainless steel fittings with an elastic clamp to immobilize the construct. Standard barb sizes accommodate .8, 1, 1.5, 2, 3, 4, 5 and 6mm lumen internal diameters and are interchangeable using a universal connector system.

CardioGen Chambers

The CardioGen bioreactor chamber accommodates pulsatile and shear stress stimulation for a single valve construct. Standard construct sizes may range from 9 to 21 mm inner diameter and from 50 to 80 mm long. Grip placement can accommodate both pulmonary valves with straight downstream vessels and aortic valves with angled downstream vessels. The modular design facilitates scaffold installation and cell seeding separate from the stimulation system. Multiple ports are available for media access, catheter probe insertion, sample collection, or further instrumentation. The autoclavable chamber has a volume of 430 mL. Additional chamber features include:

Design:
Polycarbonate hinged windows for easy viewing and laser micrometer measurement
Size:
145mm(5.7”)L x 105mm(4.15”)W x 74mm(2.9”)D
Ports:
6

 

CardioGen Grip Options

CardioGen chambers use barbed stainless steel fittings and either sutures or clamps to fix the construct in place. Standard barb sizes range in size from 9 to 21 mm and are interchangeable using a universal connector system.

Bioreactor Stimulator:

The LumeGen bioreactor system includes the 6Vr pulsatile pressure mechanical stimulator. The 6Vr stimulator features a 40N linear motor that is compact, corrosion resistant and incubator compatible. The stimulator employs pressure control to deliver user defined sinusoidal or physiological pressure waveforms to a maximum pressure of 200 mmHg. The 6Vr is integrated with a computer controlled mean flow pump and is capable of low or high-flow configurations. The flow rate of the mean flow pump is automatically altered to maintain the desired mean pressure within the heart valve. The stimulator dimensions are as follows:

Size:
152mm(6.0”)H x 102mm(4.0”)W x 102mm(4.0”)D
Weight:
2.1kg (4.6lb)

 

GrowthWorks® Software & Control:

The GrowthWorks Software and Control platform includes advanced capabilities, such as multiple waveform control, data acquisition and multi-motor operation. The intuitive software runs on a Windows® laptop computer and features a simple user interface. User defined stimulation profiles are controlled by the software and are readily monitored using graphical displays. Integrated data acquisition routines capture and record data at user prescribed intervals. The GrowthWorks Software is configurable for running four stimulators while simultaneously monitoring up to 16 transducers, allowing the researcher maximum flexibility.

The GrowthWorks Control Hardware features an embedded Intel CPU, integrated data acquisition and integrated motor drives. Communications between the laptop and control hardware is transmitted over a standard network cable. Primary advantages of the TGT Laptop/Controller approach are reduced physical size and easy upgradability. As future Windows versions or higher performance Laptops become available, either can be replaced without any software or controller changes. This ensures the GrowthWorks control system will be an integral part of your research for many years to come.

GrowthApps Stiffness Measurement & Adaptive Control:

The GrowthApps modules provided added capability to GrowthWorks. The addition of the Stiffness Measurement & Adaptive Control Application enables the system to make real-time measurements of the sample stiffness during culture. Described in U.S. Patent 7,410,792 B2 Instrumented bioreactor with material property measurement capability and process-based adjustment for conditioning tissue engineered medical products., the Application measures the mechanical response of the tissue construct as it is being stimulated and conditioned. The stiffness measurements can be used as guidelines for bench marking progress during culturing or for modifying the stimulation profile. Based upon the measured response, the bioreactor system is then capable of altering the stimulation profile. This technology enables researchers to automatically culture the tendon or ligament from initial seeding to an implantation-ready state with minimal operator intervention.

LumeGen Bioreactor Configurations

LumeGen bioreactorc configurations range from a single chamber to an integrated six chamber system. The 1V system is a single chamber design that is able to support basic pulsatile pressure and shear stress experiments. Optional features can be added including laser micrometers, pressure sensors and other transducers. The LumeGen 6Vc system integrates up to six chambers,individual reservoirs, pumps and stimulator onto a frame that can be easily placed into an incubator. Individual flow loops are provided for each chamber. The table below describes the features of the 1V and 6Vc systems.

 

VASCULAR
Key: S=Standard, A=Available, - = not available
CHAMBERS 1V 6Vc
Individual vascular growth chamber S S
6 parallel vascular growth chambers - S
Sample grips for 3, 4, 5, 6 mm lumen diameter S S
External lumen instrumentation ports per chamber 3 3
Internal lumen instrumentation ports per chamber 2 2
O-ring style sample fixation A A
Patent pending grip lock during sample handling S S
Lumen/ablumen volume isolators/reducers for scaffold seeding A A


STIMULATION    
Static pressure control (200 mm Hg peak pressure) A A
Pulsatile pressure control (sinusoidal) A S
Pulsatile pressure control (physiological content, user defined waveform) A S
Axial distension stimulation A A


CONTROLS    
Controller software, computer, and monitor S S
Power supplies and cabling S S
Mean cell culture media flow control A S
Dynamic cell culture media flow control A S


TISSUE MONITORING    
Non-contact radial or axial distension measurement A A
Non-contact flow sensors A A
Catheter pressure transducers A A


ACCESSORIES    
Media reservoir S S
Incubator A A


BENEFITS    
All-autoclavable components S S
Excellent sample visibility S S
Media change without opening reservoir via luer fittings S S
Easy rotisserie cell seeding owing to cylindrical chamber frame S S
Light weight design for easy handling S S
Transparent opposing bioreactor walls for optical inspection or measurement S S
Compact design to fit in standard incubators S S
Easily cleaned surface finishes S S
100% satisfaction guaranteed warranty S S

 

Download the 2015_LumeGen

ABOUT BISS TGT

Did you know that BISS TGT has a wide range of products for Tissue Engineering and Regenerative Medicine (TERM) Applications? Our innovative material testing technology is the most comprehensive commercial solution for the TERM market! We are proud to present BISS TGT TERM.

SERVICE & SUPPORT

OUR CUSTOMERS

  • Children's Mercy Hospital and Clinics
  • Cook Biotech
  • Drexel University
  • FDA Center for Devices and Radiological Health
  • Gel-Del Technologies
See Full List

LATEST NEWS

  • The Challenges of Tissue Characterization
    05.27.2014

    The Challenges of Tissue Characterization TERM research is poised to change the way we know health care. This cutting-edge technology demands new tools and solutions that are able to keep up with the development in personalized medicine. Instron® is delivering those solutions.

    More Info
  • BISS featured in THE HINDU
    07.23.2015

    Bangalore Integrated System Solutions (BISS), a leading test equipment manufacturer, prides in not just ‘making in India’ but also in engaging Indian minds and hands for the same. BISS is a Bengaluru-based developer, manufacturer and exporter of digitally-controlled precision test systems for material and component characterisation in industry, R&D and academia.

    More Info
  • As Featured in Quality Magazine
    10.15.2014

    All parties recognize the importance of developing test standards and providing the est possible medical devices, but determining the best methods is a challenge.

    More Info
  • Instron TERM - Video
    10.01.2014

    See the magic of Instron TERM characterize tissue and culture cells.

    More Info
  • Biomedical Advances
    11.19.2014

    Instron TERM's quarterly newsletter is focused on research and product development challenges in the tissue engineering and regenerative medicine field. Topics include new customer applications, new Instron products, and technology innovation updates.

    More Info
  • Instron TERM sponsors ISACB Webinar Series
    01.08.2015

    Register today for the Winter 2015 Webinar on February 5, 2015. "Stem Cell Therapy for Cardiovascular Disease: From Cell to Heart" Robert Deans, PhD & Keith March, MD, PhD

    More Info
Bangalore Integrated System Solutions (P) ltd.
+91 80 28360184 | tgt@biss.in
Copyright © 2015 - 2017 Bangalore Integrated System Solutions (P) ltd. . All Rights Reserved. BISS aquired Tissue Growth Technologies (TGT)